Composite

Part:BBa_K1460005:Design

Designed by: Eric Holmes   Group: iGEM14_Cornell   (2014-08-26)


Anderson Promoter + RBS + CBP4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 684
    Illegal BamHI site found at 279
    Illegal BamHI site found at 371
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Anderson promoter will provide strong constitutive expression of the CBP4 gene in E. coli.


Source

Anderson promoter is that of part BBa_J23102. CBP4 is a naturally found coding sequence in Nicotiana tabacum.[1] This part was synthesized by BioBasic and was transferred into pSB1C3 using standard cloning procedures.

References

[1] Arazi, T., Sunkar, R., Kaplan, B., & Fromm, H. (1999). A tobacco plasma membrane calmodulin-binding transporter confers Ni2 tolerance and Pb2 hypersensitivity in transgenic plants. The Plant Journal, 171-182